A simple, rapid, economical, precise and accurate stability indicating RP-HPLC method for simultaneous estimation of rosiglitazone and glimepiride in their combined dosage form has been developed. The separation was achieved by LC-20 AT C18 (150 mm×4.6 mm×5 μm) column and Water (pH 4): Methanol : Triethylamine (25:75:0.05) as mobile phase, at a flow rate of 1 ml/min. Detection was carried out at 235 nm. Retention time of rosiglitazone and glimepiride were found to be 3.253 min and 6.613 min, respectively. The method has been validated for linearity, accuracy and precision. Linearity observed for rosiglitazone 20-60 μg/ml and for glimepiride 5-15 μg/ml. The percentage recoveries obtained for rosiglitazone and glimepiride were found to be in range of 99.613 -101.017 and 99.133 -100.437 respectively. Developed method was found to be accurate, precise and rapid for simultaneous estimation of rosiglitazone and glimepiride in their combined dosage form.
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